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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Nature. 2020 Feb 5;578(7796):627–630. doi: 10.1038/s41586-020-1995-4

Figure 3. Engineering T4 hormone synthesis by bacterial maltose binding protein (MBP).

Figure 3

a) rTG mutants with only one site active at a time. T4 release measured with the same T4 ELISA as in Figure 2c. The sum of T4 produced by the individual sites recapitulates the T4 produced by WT. b) T4 ELISA after in vitro iodination of engineered MBP to reconstruct TG's hormonogenic sites. See text and Supplementary Video 2 for details. An MBP version adding a flexible SGSDYS tail to the C-terminus shows activity comparable to a single site on TG as shown in panel a). All measurements done in triplicate. Bar plot and error bars indicate average and standard deviation.