Extended Data Figure 4. Validation of TG's three-dimensional architecture by MS crosslinking.
a) Size-exclusion chromatograms of rTG before and after BS3 crosslinking and subsequent SDS-PAGE (Coomassie stained). b) Negative staining micrograph of crosslinked rTG, showing the absence of higher-order structures caused by unwanted inter-dimer crosslinks. c) Plot representing experimental crosslinks (circles) overlapping with predicted crosslinks, calculated from the structure determined here. d) - f) Detail of key TG interfaces confirmed by the crosslinking.