Extended Data Table 1. Thyroglobulin (TG) cryo-EM and model statistics.
| Data collection and processing | eTG (C2 map) | rTG (expanded map) |
|---|---|---|
| Magnification | 134,615x | 105,000x |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e-/Å2) | 45 | 40 |
| Defocus range (μm) | 0.5-3.5 | 0.5-3.5 |
| Pixel size (Å) | 1.043 | 1.149 |
| Symmetry imposed | C2 | C1 |
| Initial particle images | 422,087 | 240,194 |
| Final particle images | 181,830 | 151,601 |
| Map resolution (Å) – (FSC 0.143) | 3.39 | 3.67 |
| Map resolution range (Å) | 2.8-7.0 | 3.3-5.0 |
| Map sharpening B factor (Å2) | -138.641 | -113.164 |
| Refinement | ||
| Model resolution (Å) (FSC 0.5) | 3.4 (3.8) | |
| PDB ID | 6SCJ | |
| EMDB | EMD-10141 | |
| Model composition | ||
| Non-hydrogen atoms | 39,886 | |
| Protein residues | 2,569 | |
| RMS deviations | ||
| Bond lengths (Å) | 0.005 | |
| Bond angles (°) | 1.274 | |
| Validation | ||
| Molprobity score (percentile) | 1.85 (83rd) | |
| Clashscore | 4.88 | |
| Poor rotamers (%) | 0.14 | |
| Ramachandran plot | ||
| Favored (%) | 86.69 | |
| Allowed (%) | 13.31 | |
| Disallowed (%) | 0 | |