Figure 5.
LOX10‐yellow fluorescent protein (YFP) tagged maize lines reveal that LOX10 localizes to chloroplasts of bundle sheath cells. (a)–(d) Images of untransformed leaves of B73 inbred line, (e)–(l) images of transgenic lines in the B73 background expressing YFP‐tagged LOX10 (LOX10‐YFP) under its native promoter, where (e)–(h) show images comparable to (a)–(d) and (i)–(l) show zoomed‐in views of (e)–(h) detailing LOX10 localization to bundle sheath chloroplast (i)–(l). Columns in order of left to right show brightfield views, chlorophyll autofluorescence, YFP fluorescence, and a merged view. Scale bars = 50 µm