Skip to main content
. 2020 Feb 27;21(5):686–701. doi: 10.1111/mpp.12922

Figure 3.

Figure 3

Overexpression of CAS enhances resistance of Arabidopsis to Sclerotinia sclerotiorum. (a) The wild‐type plant Col‐0 and CAS overexpression plants 35S:AtCAS‐1 and 35S:AtCAS‐2 were challenged with S. sclerotiorum strains Ep‐1PNA367 (top) and A10 (bottom). Photographs were taken at 36 hours post‐inoculation (hpi). (b) Statistical analysis of the lesion area induced by Ep‐1PNA367 or A10 on each plant at 36 hpi. Values are means ± SE. (c) The relative expression levels of the salicylic acid (SA) signalling pathway‐related genes (ICS1, PAD4, PBS3, EDS5, and PR1) in each plant were analysed at 12 hpi. The expression levels of GAPDH were used to normalize the expression levels of these genes in the different samples. The expression level in the wild‐type plant Col‐0 without inoculation was set as 1. Values are means ± SD. (d) SA concentrations in each plant were measured at 12 hpi. The wild‐type plant Col‐0 without inoculation was used as control. In all experiments, three independent replicates were performed. Values are means ± SE. Different letters on the same graph indicate statistical significance (one‐way analysis of variance; post hoc = Duncan α [0.05])