Fig. 2.
Results obtained from analyses of ABA and its metabolites (a–c) and expression of ABA metabolism- and signal transduction-related genes in healthy (H) and berry shrivel (BS) grape clusters collected at six sampling dates (EL32, EL33, EL34, EL35, EL36/1, EL36/2, EL37) (d–g). a Abscisic acid (ABA) content, b phaseic acid content (DPA) and c ABA-glucose ester content (ABA-GE) in berry samples collected 2011. Expression of ABA biosynthetic genes genes VviNCED2 (d) and VviNCED3 (e) and ABA-metabolic gene VviUGT73B4 (f) determined by qPCR (samples 2011) in comparison with RNAseq analyses (samples 2013). g RNAseq results on genes related to ABA biosynthesis, metabolism, signaling, and responses in BS samples shown as logFC. All data are mean values ± standard error (n = 3 each).Veraison (V) is defined as the start of coloring (EL35) and first BS symptoms were determined at EL36/1. Statistical significant differences are indicated with an asterisk (2011) and a plus symbol (2013) (P < 0.05)