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. 2020 Apr 20;11:1892. doi: 10.1038/s41467-020-15751-6

Fig. 8. African Serotype 1 causes significant increases to human primary alveolar epithelial cell barrier permeability.

Fig. 8

Assessment of epithelial cell barrier permeability changes during infection with different S. pneumoniae serotypes was performed using fluorescein isothiocyanate (FITC)-dextran (70 kDa, 1 mg per ml), see methods for full details. White bars represent FITC-Dextran emissions from untreated cells. Error bars represent mean ± SEM. Statistical analysis was performed using Two-way ANOVA and Tukey’s multiple comparison test. a Comparison of FITC-Dextran excitation emission between serotype 2(D39) (black bars) and 1 (ST217S) (red bars). ****P-value < 0.0001. b Comparison of FITC-Dextran excitation emission between serotype 5 (diagonally hashed bars) and 1 (ST217S) (red bars). *P-value = 0.0178, ***P-value = 0.0007, ****P-value < 0.0001. c Comparison of FITC-Dextran excitation emission between serotype 1 (ST306) (horizontal bars) and 1 (ST217S) (red bars). ****P-value < 0.0001. d Comparison of FITC-Dextran excitation emission between serotype 1 (ST217S) infection with (red, diagonally hashed bars) and without (red bars) the addition of liposomes. ****P-value < 0.0001. As a control, cells were also treated with liposomes in the absence of bacteria (black bars, second column). Source data are provided as a Source Data file.