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. 2020 Apr 20;11:1886. doi: 10.1038/s41467-020-15809-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Heatmap of heat-activated TEs in Control, Heat, and Recovery. The chromatin track shows the genomic locations of heat-activated TEs. The class track shows the three classes of the heat-activated TEs. The expression track shows the expression levels of heat-activated TEs in log₂ (CPM + 1). The bottom three tracks show relative expression levels (Z-scores) for Control, Heat, and Recovery and range from −2 (blue) to 2 (red). Most heat-activated TEs, classified into Group 1, are silenced again during recovery. Some heat-activated TEs, classified into Group 2, exhibited persistent activation or were not completely restored during recovery. b Expression dynamics of randomly selected Group 1 and Group 2 TEs as determined by RT-qPCR. The expression value of each replicate was normalized to the expression level of Col-0. Circles denote relative expression values. Bars indicate mean values of three technical replicates. c The enrichment (red) and depletion (blue) patterns of epigenetic features in Group 1 and Group 2 TEs compared with total TEs in Arabidopsis. Relative density ranges from −2 to 2. Gray indicates that the epigenetic feature is neither enriched nor depleted (two-sided Mann–Whitney U test, p > 0.05). Source data underlying b and c are provided as a Source Data file.