Fig. 5. Bryostatin-induced PKC translocation.

a Bryostatin-induced translocation of PKC from the cytosol to the membrane was determined in real time by monitoring cytosolic fluorescence of a PKCδ-GFP fusion protein using confocal microscopy. Scale bar represents 25 μm. Experiments performed in n = 3 biological replicates. b–d Cytosolic fluorescence normalized to t = 0 (time immediately prior to addition of compound to media) and plotted against time. Error bars excluded for clarity (data presented as the mean of n = 3 biological replicates). Individual curves with error bars included in Supplementary Information. Maximum translocation of PKCδ-GFP to the plasma membrane reported in Table 1.