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. 2020 Apr 20;11(4):253. doi: 10.1038/s41419-020-2449-5

Fig. 5. Dual inhibition of FAO and glycolysis elicits necroptosis-mediated metabolic synthetic lethality in MES cells in vitro.

Fig. 5

a MES83 and b 1027 A cells were +/– pretreated with necrostatin-1 (Nec-1; 100 µM, 30 min) and then treated with +/− etomoxir (40 µM), 2DG (35 mM) or the combination (n = 3). Non-viable cells were counted with trypan blue after 48 h. c Mitochondrial superoxide was evaluated with MitoSOX Red after 48 h. treatment with +/− etomoxir (40 µM), 2DG (35 mM) alone or in combination (n = 4). Expression of molecules involved in necroptosis was analyzed by western blot (d) after 48 hr treatment with indicated inhibitors. Normalized expression of e RIP1 (n = 4) and f pMLKL (n = 5) was quantified by densitometry. Line between the data points represents mean. *p < 0.05; **p < 0.005; ***p < 0.0005.