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. 2020 Apr 20;11(4):248. doi: 10.1038/s41419-020-2471-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The inhibitory activities of sulfathiazole (SFZ) against human SPR were measured in a spectrophotometric assay. b The effects of SFZ on cell viability of SMMC-7721 and BEL-7402 cells were detected using colony formation assay. c Cell apoptosis was detected by flow cytometry after treatment with SFZ at the indicated concentrations for 96 h in SMMC-7721 and BEL-7402 cells. d The effect of BH₄ on cell apoptosis of SMMC-7721 and BEL-7402 cells transfected with siSPR. e The enzymatic activity of SPR in the wild type (WT) and indicated SPR mutant at different protein amount titrations in the biochemistry enzymatic assay. f The point mutant of SPR in SMMC-7721 was confirmed by Sanger sequencing. g Apoptosis in SMMC-7721 and SMMC-7721/SPR D257G cells was analyzed by detecting the PARP cleavage and Caspase 3 cleavage level. h The colony formation assay of SMMC-7721 with the indicated mutant. Data are represented as the mean ± SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests. TAX paclitaxel.