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. 2020 Apr 20;11:1867. doi: 10.1038/s41467-020-15777-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Alternative catalytic activities of SaDAH-WT and SaDAH-G226D. The chlorination, hydroxylation and azidation activities are colored in blue, red, and yellow, respectively. The putative regiochemistry of the −OH and −N₃ in 7 and 8 are highlighted in red, while the stereochemistry of the products remains undetermined. b XICs showing the in vitro activity of SaDAH-WT that chlorinates 5 to yield 1 in a 2OG-dependent manner. c XICs showing the in vitro activity of SaDAH-G226D that predominantly hydroxylates 5–7 in a 2OG-dependent manner. The chlorination product 1 is also produced as a minor product. d XICs showing the 2OG-dependent azidation activity of SaDAH-WT that converts 5–8 in reaction buffer containing 1 mM NaN₃. The chlorination product 1 and the hydroxylation product 7 are also produced as side products. Mass windows used for displaying the XICs: 5, 364.176 m/z; 1, 398.137 m/z; 7, 380.17 m/z; and 8, 405.18 m/z.