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. 2020 Apr 20;11(4):252. doi: 10.1038/s41419-020-2460-x

Fig. 1. Carnosol inhibits NLRP3 inflammasome activation triggered by nigericin in BMDMs, THP1, and PBMCs.

Fig. 1

a Western blot analysis of caspase-1 (p20) and IL-1β in culture supernatants (Sup.) and pro- IL-1β, caspase-1 (p45), NLRP3 and ASC in cell lysates (Lys.) of LPS-primed BMDMs treated with various doses of carnosol and then stimulated with nigericin. b–e Activity of caspase-1 (b), ELISA of IL-1β (c), release of LDH (d) and ELISA of TNF-α (e) in Sup. from samples described in a. f Western blot analysis of caspase-1 (p20) and IL-1β in Sup. and pro- IL-1β, caspase-1 (p45), NLRP3 and ASC in cell Lys. of PMA-primed THP1 treated with various doses of carnosol and then stimulated with nigericin. g–i Activity of caspase-1 (g), ELISA of IL-1β (h) and TNF-α (i) in Sup. from samples described in f. j, k Activity of caspase-1 (j) and ELISA of IL-1β (k) in Sup. from LPS-primed hPBMCs treated with various doses of carnosol and then stimulated with nigericin. Coomassie blue staining was used as the loading control in Sup. GAPDH served as a loading control in the Lys. Data are represented as the mean ± SD from at least four biological samples. The significance of the differences was analyzed using Mann–Whitney U test: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control, NS, not significant.