Figure 6.

Selective deletion of Pyk2 in D1R-expressing cells blunts the acute locomotor response to a D1R agonist, but not to an anticholinergic agent. A-B, Locomotor activity of Pyk2^f/f (n = 20 mice) and Pyk2^f/f;D1::Cre mice (n = 27 mice) after a first (A) and a second (B, 13 days later) injection of the D1 agonist SKF-81297 (SKF, 3 mg/kg, arrow) injected 30 min after mice were placed in the open field. In A the locomotor activity was lower in Pyk2^f/f;D1::Cre mice than in Pyk2^f/f mice (2-way ANOVA, genotype effect p < 0.05 (Supplementary Table 1). (C), Time spent grooming was quantified as an index of stereotypies 35–40 min and 40–45 min (periods 1 and 2, respectively, indicated by an horizontal line in A) after the beginning of the recording in 6 randomly selected mice of each genotype. Since there was no difference between the two genotypes, data were pooled. (D), Pairwise comparison of the distances traveled during the 45 minutes following the first and the second SKF injections in Pyk2^f/f and Pyk2^f/f;D1::Cre mice (data from A and B). (E), Sensitization ratios, as in Fig. 3F, during the 45 min after SKF injection in the two genotypes (data from D). (F-G), Specific deletion of Pyk2 in D1 neurons does not alter the locomotor responses to an anticholinergic drug, trihexyphenidine (THX). Locomotor activity of Pyk2^f/f (n = 12 mice) and Pyk2^f/f;D1::Cre mice (n = 13) after a first (F) and, 13 days later, a second (G) injection of THX (15 mg/kg i.p., arrow) injected 30 min after mice were placed in the open field. Data in A, B, D, F, and G were analyzed with 2-way ANOVA and post-hoc Sidak’s multiple comparisons test, those in C and E with two-tailed unpaired Students t-test. ns, not significant, *p < 0.05, ****p  <  10⁻⁴. See Supplementary Table 1 for detailed statistical analyses.