Figure 2.

A) and B) GPC signals of Nap-Dex (blue), dextran (black) and Nap-Dex incubated with 10 mM H₂O₂ (green). Samples were incubated for 24 hours before the measurement in 80 v% DMF and 20 v% aqueous solutions. C) Nap-Dex solution with/without 10 mM H₂O₂. Nap-Dex was dissolved in 50 v% DMSO and 50 v% H O and incubated for 3 hours at 37 ° Polymer concentration was 10 mg/ml and H₂O₂ concentration was adjusted to 0 mM or 10 mM. D) Turbidity change of the Nap-Dex solution measured by UV-Vis spectrometer. Nap-Dex solution (2 mg/ml in 50 v% DMSO and 50 v% aqueous) was incubated at 37 °C and the absorption at 750 nm was monitored by incubation time. E) LC-MS comparison between released molecules from Nap-Dex by H₂O₂ and pure naproxen. The Nap-Dex solution incubated with 10 mM H₂O₂ was filtrated using ultracentrifugal filtration and filtrated molecules were injected into LC-MS. F) Naproxen release from Nap-Dex by H₂O₂. The naproxen signal in LC-MS was monitored by incubation time. The release amount was calculated based on a calibration curve.