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. 2019 Nov 20;43(2):487–506. doi: 10.1007/s10753-019-01132-9

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Genome-wide microarray analysis of BMDMs incubated with MTB Hsp16.3. Bone marrow cells were isolated from the tibias and femurs of BALB/c mice (6–8 weeks old) and incubated with 20 ng/ml GM-CSF for 7 days. Then, BMDMs were treated with 100 ng/ml MTB Hsp16.3 for 72 h, and the cells were collected. Global gene expression was analyzed by a cDNA chip array. a Heat map. b Scatterplot of gene expression. c The fold change and frequency. d Prediction of 5 target genes, including AHR, CCR2, CCRL2, CX3CR1, and Mertk. e BMDMs were treated with 100 ng/ml MTB Hsp16.3 for 72 h, cells were collected, and the mRNA expression of the indicated genes was determined by real-time PCR. f Cells were harvested in RIPA buffer containing protease and phosphatase inhibitor cocktails, and the protein expression levels of CCRL2 and CX3CR1 were determined by Western blot. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (0 h group).

Fig. 2 — Genome-wide microarray analysis of BMDMs incubated with MTB Hsp16.3. Bone marrow cells were isolated from the tibias and femurs of BALB/c mice (6–8 weeks old) and incubated with 20 ng/ml GM-CSF for 7 days. Then, BMDMs were treated with 100 ng/ml MTB Hsp16.3 for 72 h, and the cells were collected. Global gene expression was analyzed by a cDNA chip array. a Heat map. b Scatterplot of gene expression. c The fold change and frequency. d Prediction of 5 target genes, including AHR, CCR2, CCRL2, CX3CR1, and Mertk. e BMDMs were treated with 100 ng/ml MTB Hsp16.3 for 72 h, cells were collected, and the mRNA expression of the indicated genes was determined by real-time PCR. f Cells were harvested in RIPA buffer containing protease and phosphatase inhibitor cocktails, and the protein expression levels of CCRL2 and CX3CR1 were determined by Western blot. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (0 h group).