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. 2019 Nov 20;43(2):487–506. doi: 10.1007/s10753-019-01132-9

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — CCRL2/CX3CR1 overexpression promotes the macrophage M2 phenotype. a The schematic structure of pcDNA3.1-CCRL2 (termed p-CCRL2) and pcDNA3.1-CX3CR1 (termed p-CX3CR1). b Identification of pcDNA3.1-CCRL2 and pcDNA3.1-CX3CR1 by BamHI and HindIII double enzyme digestion. c DNA sequence of the recombinant plasmid. BMDMs were transiently transfected with p-CCRL2, p-CX3CR1, or p-cont in vitro and then incubated with 100 ng/ml MTB Hsp16.3 for 0–48 h. d The mRNA expression levels of IL-10, Arg-1, and TGF-β were determined by real-time PCR. e The production of NOS2 or CD206 in F4/80-positive macrophages was measured by FCM. f The secretion levels of IL-10, TGF-β, and TNF-α were measured by ELISA at 48 h. The data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (0 h group).

Fig. 4 — CCRL2/CX3CR1 overexpression promotes the macrophage M2 phenotype. a The schematic structure of pcDNA3.1-CCRL2 (termed p-CCRL2) and pcDNA3.1-CX3CR1 (termed p-CX3CR1). b Identification of pcDNA3.1-CCRL2 and pcDNA3.1-CX3CR1 by BamHI and HindIII double enzyme digestion. c DNA sequence of the recombinant plasmid. BMDMs were transiently transfected with p-CCRL2, p-CX3CR1, or p-cont in vitro and then incubated with 100 ng/ml MTB Hsp16.3 for 0–48 h. d The mRNA expression levels of IL-10, Arg-1, and TGF-β were determined by real-time PCR. e The production of NOS2 or CD206 in F4/80-positive macrophages was measured by FCM. f The secretion levels of IL-10, TGF-β, and TNF-α were measured by ELISA at 48 h. The data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (0 h group).