Differences in Ultrastructure of Wing Pads Developing into Short or Long Brown Planthopper Wings
(A and B) (A) Whole wing pads were dissected 95 h post-molt to fifth instar, and actin stained with phalloidin-iFluor 488 (green) and nuclei stained with DAPI (blue). White rectangles indicate regions that are further magnified in (B). (B) Enlargement of (A) showing the distal portion of the wing pad with the microvilli-like epithelium in long-winged individuals; arrows indicate the differential structures in the long and short wings.
(C) Transmission electron microscopic image of the wing pads of the fifth-instar nymphs (6000X). SW, short-winged development induced by knockdown of InR1; LW, long-winged induced by knockdown of FOXO.