Figure 7.
Effects on Wing Morph and Transcription of Cell Division-Related Genes After Injection of FOXO dsRNA and/or Paclitaxel
(A) Fourth-instar nymphs received injections of dsGFP or dsFOXO, followed by the second injection of paclitaxel (PTX) or water at the fifth-instar nymph stage. dsFOXO + PTX (n = 68 females, 43 males), dsFOXO + H2O (n = 37 females, 41 males), dsGFP + PTX (n = 31 females, 27 males), dsGFP + H2O (n = 32 females, 27 males).
(B) Fourth-instar nymphs received injections, wing buds were subsequently dissected, and relative expression of the genes E2F3, E2F5, E2F6, E2F7, E2F8, CDK1, CDK2, DP1, DP2, CCNE, Futsch, and PCDH15 were measured by RT-PCR. Chi-square test was used in (A). ∗∗∗p < 0.001. Twelve biological replicates were used for each treatment. Treatments found to be significantly different by Duncan's multiple range-test (p < 0.05) are indicated by different letters (B). Data are represented as mean ± SD.