Table 2.
Counts of peptides in the digests of 4 actomyosin fractions (myosin, actin, tropomyosin, myosin binding protein C) after different salting treatments
| Samples | Number of identified peptides in digests | |||
|---|---|---|---|---|
| myosin | tropomyosin | actin | Myosin binding protein C | |
| Before heat treatment | ||||
| 0.4 M (without DTT) | 24 | 8 | 4 | 5 |
| 0.6 M (without DTT) | 59 | 12 | 9 | 9 |
| 0.8 M (without DTT) | 28 | 10 | 6 | 8 |
| 0.4 M (with DTT) | 25 | 8 | 4 | 10 |
| 0.6 M (with DTT) | 53 | 17 | 9 | 15 |
| 0.8 M (with DTT) | 49 | 13 | 8 | 10 |
| After heat treatment | ||||
| 0.4 M (without DTT) | 53 | 12 | 9 | 14 |
| 0.6 M (without DTT) | 69 | 12 | 10 | 18 |
| 0.8 M (without DTT) | 60 | 15 | 9 | 14 |
| 0.4 M (with DTT) | 94 | 10 | 10 | 23 |
| 0.6 M (with DTT) | 137 | 19 | 14 | 33 |
| 0.8 M (with DTT) | 99 | 14 | 13 | 24 |