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. 2020 Apr 14;11:331. doi: 10.3389/fphys.2020.00331

FIGURE 1.

FIGURE 1

MiR-29c is an important mediator of TGF-β1-induced renal fibrosis in NRK-49F cells. RT-PCR results of miR-29c expression in NRK-49F cells treated with different dosages of TGF-β1 (A) or over different culture durations with TGF-β1 (B). RT-PCR results of expression levels of several fibrosis-related genes (α-SMA, HGF, fibronectin, and Col3a1) in NRK-49F cells infected with miR-29c mimic (C) or miR-29c inhibitor (D) followed by 12 h of PBS or TGF-β1 (10 ng/mL) treatment. Representative western blots show levels of α-SMA, HGF, fibronectin, and Col3a1 proteins in NRK-49F cells infected with miR-29c mimic (E) or miR-29c inhibitor (F) followed by 12 h of PBS or TGF-β1 (10 ng/mL) treatment. Protein expression was normalized with β-actin. Data presented are mean ± SEM values. Symbols (“∗” and “#”) represent statistical significance.