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. 2020 Apr 14;11:331. doi: 10.3389/fphys.2020.00331

FIGURE 4.

FIGURE 4

Reciprocal regulation of miR-29c and the Wnt/β-catenin pathway in renal fibroblasts. Representative western blots show levels of TGF-β1, p-GSK3β, GSK3β, p-β-catenin, and β-catenin proteins in kidneys transfected with pre-miR-29c (A) or miR-29c inhibitor (B) 10 days after the sham procedure or UUO surgery. Protein expression was normalized with β-actin. (C) Quantitative analysis of the protein levels in (A). (D) Quantitative analysis of the protein levels in (B). Representative western blots show protein levels of TGF-β1, p-GSK3β, GSK3β, p-β-catenin, and β-catenin in NRK-49F cells infected with miR-29c mimic (E) or miR-29c inhibitor (F) followed by a 12-h PBS or TGF-β1 (10 ng/mL) treatment. (G) Quantitative analysis of the protein levels in (E). (H) Quantitative analysis of the protein levels in (F). Representative photomicrographs of NRK-49F infected with miR-29c mimic (I) or miR-29c inhibitor (J) followed by a 12-h PBS or TGF-β1 (10 ng/mL) treatment, staining for β-catenin, and counterstaining with DAPI (blue). The data are presented as mean ± SEM values. Symbols (“∗” and “#”) represent statistical significance.