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. 2020 Apr 20;11:1910. doi: 10.1038/s41467-020-15633-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Principal component analysis (PCA) based on the complete metabolomics dataset. Samples are colored according to cultivar, and shapes refer to the position in the leaf. b Biosynthesis pathway of benzoxazinoids. Compounds with significant differences in the comparison between cultivars are highlighted in orange (Obelisk) or green (Chinese Spring). nd not detected, ns not significant. c Compounds of the biosynthesis pathway of benzoxazinoids with significant differences at local site of inoculation with Z. tritici (Zt) IPO323 or mock (m) at 4 and 8 dpi-f between wheat cultivars Obelisk and Chinese Spring. Active forms of benzoxazinoids are highlighted in blue. d Significantly different compounds in the benzoxazinoid biosynthesis pathway at an adjacent site to the inoculation in (c). Active forms of benzoxazinoids are highlighted in blue. Number of biologically independent replicates: n = 3. For details on the statistical analysis of the metabolomics dataset, please see the methods section. *P < 0.05; **P < 0.01; ***P < 0.001.