Fig. 3. Comparative genomic analysis of PTs and the CDX and the CDX-derived cell line.

a Numbers of mutations according to their recurrence in PT specimens. A total of 205 mutations were detected in PT specimens. In total, 153 (75%) mutations were detected in one PT, 27 were detected in two PTs, 9 were detected in three PTs, 8 were detected in four PTs, 5 were detected in five PTs, 2 were detected in six PTs, and one was detected in seven PTs. b Shared mutations between PTs, CTCs, and CDX and cell line. Of the 205 mutations detected in PTs, 172 were found only in PTs, 32 were shared between PTs, the CDX and the cell line and one mutation was shared between PTs and CTCs but not with the CDX and the cell line. c Heatmap of the 32 PT mutations shared with CTCs, the CDX and the cell line. Mutated genes, amino acid changes, and mutations status in the 16 tumor samples including two TURP, six biopsies, the CDX, the cell line, and six CTC samples are indicated. Red color indicates that the sample is mutated, green indicates that the sample is not mutated, and white indicates that the position is not covered with a sufficient depth of sequencing. d Percentage of mutations of each PT shared by the CDX. For each PT the number of mutations is considered as 100%. The percentage of shared mutations between each PTs and the CDX is represented in pink. Mutations present only in PTs are represented in blue. e Shared CNAs between PTs, the CDX and the cell line. Five of the six CNAs found in PTs are shared with CTCs, the CDX. f Heatmap of the five PT CNAs shared with CTCs, the CDX and the cell line. Chromosomes harboring CNAs, CNA status in the 16 tumor samples and candidate driver genes in chromosome segments are presented.