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. 2020 Apr 20;11(4):258. doi: 10.1038/s41419-020-2442-z

Fig. 3. COL11A1 activates AMPK through DDR2-Src-Akt-dependent signaling.

Fig. 3

a, b Western blot of pAMPK (T172) and CPT1A in ES2 (a) or OVCAR3 (b) cells cultured in either PBS or COL11A1-coated plates for 48 h and treated with dorsomorphin (DM) at 8 µM for 48 h. GAPDH was used as a loading control. N = 2. c, d Western blot of pAMPK (T172) in ES2 cells expressing either scrambled or shDDR2 (c) and shITGA1 (d) cultured on either in PBS or COL11A1-coated plates for 48 h. GAPDH was used as a loading control. N = 2. e, f Western blot of pAMPK (T172) in ES2 (e) or OVCAR3 (f) cells cultured in either uncoated or COL11A1-coated plates for 48 h and treated with Dasatinib (2 µM for ES2 and 5 µM for OVCAR3) for 48 h. GAPDH was used as a loading control. N = 2. g Western blot of pAMPK (T172) in ES2 cells cultured in either PBS or COL11A1-coated plates for 48 h and treated with LY294002 (20 µM) for 48 h. GAPDH was used as a loading control. N = 2. h Western blot of pAMPK (T172) in OVCAR3 cells cultured in either PBS or COL11A1-coated plates for 48 h and treated with MK2206 (20 µM) for 48 h. GAPDH was used as a loading control. N = 2.