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. 2020 Apr 20;10:6591. doi: 10.1038/s41598-020-63245-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM images of non-irradiated particles, (a) AuNPs and (c) AuPEG₂₀₀₀-NH₂, in comparison with irradiated products, (b) iAuNPs and (d) iAuPEG₂₀₀₀-NH₂. The polymer coating has a slight influence on the particle size, but showing a more significant effect on aggregation behavior. The irradiated PEGylated nanoparticles exhibit a phenomenon of uniform clustering, with the formation of entities with dimensions up to 100 nm. The histograms indicating dimensional distribution by measuring about 1000 particles from several TEM images, of which only representative ones are illustrated above.