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. 2020 Apr 20;10:6591. doi: 10.1038/s41598-020-63245-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The fluorescence emission spectra of pristine DOX at different concentrations and of DOX-PEG₅₀₀-epoxy, AuPEG₂₀₀₀-DOX, iAuPEG₂₀₀₀-DOX, AuPEG₂₀₀₀-TAT-DOX, iAuPEG₂₀₀₀-TAT-DOX at DOX concentration of around 0.2 mg/mL; (B) the fluorescence emission spectra of DOX-PEG₅₀₀-epoxy at different DOX concentrations; (C, D) the variation of the fluorescence peaks vs. DOX concentration of the studied systems at λ_em = 590 nm and λ_em = 556 nm respectively; all spectra were registered at λ_ex = 480 nm.