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. 2020 Apr 20;10:6618. doi: 10.1038/s41598-020-63588-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo experimental setup for a proof-of-concept of prostatic nerve imaging using near-infrared cyanine VSD: (a) in vivo experimental protocol. (b) The validation of no adverse effect of the direct VSD administration using intracavernosal pressure (ICP) measurements before and after the electrical stimulation in control and VSD groups. (c) Corresponding observation of erectile function at each experimental phase (Movie 1).