Fig. 2. Notch inhibitor DAPT improved transplantation efficiency.

a Experimental design-1. To evoke muscle regeneration, BaCl₂ was injected into TA muscles of NOD-scid mice 24 h before transplantation. The next day, Hu5/KD3 cells (5.0 × 10⁵ cells) were transplanted into damaged TA muscles with or without DAPT. TA muscles were isolated 4 weeks after transplantation. b Engraftment and differentiation of a human myoblast cell line, Hu5/KD3 cells, with or without DAPT. Donor cell-derived myofibers were detected as human lamin A/C (nuclear membrane)-positive and human spectrin (plasma membrane)-positive myofibers. Scale bar = 100 µm. c The number of human lamin A/C- and human spectrin-positive myofibers per view. Data were analyzed by unpaired two-tailed Student’s t-test. n = 3 mice/group. For each mouse, 4–8 muscle sections were examined. The effect size of Pearson’s r correlation (r) is shown. d Experimental design 2. hiPSC-derived muscle progenitors (ERBB3(+)CD271(+) cells) were transplanted into TA muscles of NSG-mdx^4Cv mice with or without DAPT. BaCl₂ injection and sampling of TA muscles was performed as in (a). DAPT was injected into the engrafted TA muscle four times every 3 days after transplantation. e Representative photos of engraftment and differentiation of human iPSC-derived DAPT-treated muscle progenitors. f The number of human lamin A/C- and human spectrin-positive myofibers per view. Data were analyzed by unpaired two-tailed Student’s t-test. n = 3 mice/group. For each mouse, 4–16 slices were examined. The effect size of Pearson’s r correlation (r) is also shown. In b, e, scale bar indicates 100 µm.