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. 2020 Apr 20;11(4):249. doi: 10.1038/s41419-020-2438-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Migration of 5 different cultured patient-specific HGSC tumor cells (OCMI OC_37, 38, 58, 92, 108) was analyzed using conditioned media of ascites-derived TAM from 3 different patients (TAM_169, 170, 171) as chemoattractant in a transwell assay format. Background migration was measured in the absence of any attractant (Ctr−). Data were normalized to 1 for FCS-induced migration (Ctr+) in each OCMI cell line (a). b Exemplary microscopic pictures showing migrated OCMI cells (OC_ 38, 58, 92) in response to conditioned media of TAM_170 and FCS (Ctr+) as well as background control without chemoattractant (Ctr−). c, d Conditioned media of m1 (induced by LPS+IFNγ), m2c (induced by IL-10), and asc-MDM (induced by ascites) from 6 donors were applied as attractants for migration of OCMI cell line OC_58. The corresponding data for the phenotypes of MDM differentiation are shown in Supplementary Fig. S1. Migration is expressed relative to FCS-induced migration (c) and relative to migration induced by TAM-like MDM (d). e Transwell migration format using OCMI cells (OC_58) pretreated with conditioned media of m1-MDM, m2c-MDM, and asc-MDM (3 different donors) for 17 h prior to analysis of tumor migration using FCS as chemoattractant for 2 h. As controls, untreated tumor cells were allowed to migrate in the presence (Ctr+) and absence of FCS (Ctr−). For details, see “Materials/subjects and methods.” Migration of pretreated tumor cells was expressed relative to untreated cells in the presence of FCS. Horizontal bars show the mean. Standard deviations are given. Asterisks indicate p values determined by two-sided, paired t test. *p < 0.05, ***p < 0.001.