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. 2020 Apr 20;11(4):249. doi: 10.1038/s41419-020-2438-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Expression of TGFBI mRNA in asc-MDM, m1-MDM, and m2c-MDM analyzed by RT-qPCR in five different donors. b Detection of TGFBI protein in cell lysates by western blotting. β-Actin was used as loading control. Blots of three donors are shown. c TGFBI secretion of polarized MDM measured by ELISA of conditioned media (n = 4). TGFBI protein levels are indicated as ng/ml. d Expression of TNC mRNA was analyzed in asc-MDM, m1-MDM, and m2c-MDM by RT-qPCR in five different donors. e Detection of TNC protein in cell lysates by western blotting. β-Actin was used as loading control (same blot as in b, since both TGFBI and TNC were analyzed in the same experiment) (n = 3). f Western blot of TNC protein in the conditioned media. The analysis was carried out with tenfold concentrated conditioned media from equal numbers of different MDM subtypes. g Quantification of TNC secretion by different MDM subtypes was performed using the Image LabTM 5.0 software in five different donors. TNC protein levels were normalized to 1 for asc-TAM. p Values were determined by paired t test (*p < 0.05, ***p < 0.001).