Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 20;11(4):249. doi: 10.1038/s41419-020-2438-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a TGFBI secretion by m2c-MDM and asc-MDM after siRNA-mediated TGFBI silencing. TGFBI concentration in the conditioned media of MDM transfected with control siRNA and TGFBI siRNA (pool of three siRNAs) was determined by ELISA and normalized to the untransfected control. Depicted are the data of five different macrophage preparations. Additional data of TGFBI gene expression and intracellular protein levels in TGFBI siRNA-transfected macrophages are shown in Fig. S3. b Influence of TGFBI knockdown on the migration-promoting potential of asc-MDM and m2c-MDM. OCMI tumor cells (OC_58) were pretreated with conditioned media of the untransfected and siRNA-transfected cells before applied to a transwell migration assay with FCS as attractant, as described in the legend of Fig. 6. Migration was expressed relative to the untransfected control for each of the four different macrophage preparations. Horizontal bars show the mean and two-sided, paired t test was calculated (*p < 0.05, **p < 0.01). c Representative microscopic pictures of tumor cell migration induced by conditioned media from m2c-MDM (donor 11) untransfected or transfected with control siRNA or TGFBI siRNA.