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. 2020 Apr 20;11:1896. doi: 10.1038/s41467-020-15717-8

Fig. 5. Paracrine regulation of exocytosis in dispersed α-cells.

Fig. 5

a Cumulative time course (upper), total exocytosis (middle), and initial density of docked granules (lower) during K+-stimulated (gray bar) exocytosis in dispersed ND α-cells in control conditions (black, 10 mM glucose, n = 71 cells/14 donors) or exposed to somatostatin (light blue, SST, 400 nM, n = 53 cells/9 donors), insulin (green, INS, 100 nM, n = 53 cells/8 donors), forskolin (purple, FSK, 2 µM, n = 30 cells/5 donors), GABA (brown, 400 nM, n = 14 cells/3 donors), adrenaline (pink, ADR, 5 µM, n = 30 cells/5 donors), glutamate (orange, Glut, 1 mM, n = 16 cells/3 donors). In a–d, significant differences compared with control are indicated with p-values (one-way ANOVA, Fisher posthoc test). Data are presented as mean values ±SEM. b As in A, but for dispersed T2D α-cells. T2D ctrl n = 33 cells/6 donors, T2D SST n = 26 cells/5 donors, T2D INS n = 19 cells/4 donors, T2D ADR n = 19 cells/3 donors. c, d As in a, b, but in presence of 1 mM glucose. ND ctrl n = 30 cells/6 donors, ND SST n = 27 cells/6 donors, ND INS n = 24 cells/5 donors, ND ADR n = 15 cells/3 donors, ND Glut n = 9 cells/2 donors, T2D ctrl n = 27 cells/5 donors, T2D SST n = 17 cells/3 donors, T2D INS n = 23 cells/3 donors, and T2D ADR n = 10 cells/2 donors.