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. 2020 Apr 20;11(4):256. doi: 10.1038/s41419-020-2424-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Mice with liver I/R injury were treated with PBS, 3-MA, UC-MSCs or UC-MSCs+3-MA were killed 6 h after reperfusion. a The number of autophagic vacuoles was determined by TEM. The data are presented as the means ± SEMs (n = 5/group). b Western blot assays were performed to detect the level of autophagy in liver tissues. c Representative liver sections from each experimental group stained with DHE and DAPI were obtained to determine the total ROS level. The data are presented as the means ± SEMs (n = 5/group). d Representative liver sections from each experimental group stained with MitoSOX Red and DAPI were obtained to determine the level of mtROS. The data are presented as the means ± SEMs (n = 5/group). e H&E staining was used for the assessment of liver injury (magnification ×100). The data are presented as the means ± SEMs (n = 5/group). f Representative liver sections from each group stained with caspase-3 were obtained (magnification ×200). The IHC staining of caspase-3 in liver tissues was semiquantified (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 (all p-values were obtained by one-way ANOVA).