AI-2 activity in V. harveyi BB152, E. piscicida ΔluxS, and E. piscicida EIB202 using the V. harveyi bioluminescence assay. V. harveyi BB152 served as a positive control, and E. coli DH5α served as a negative control. Data are presented as means ± SD of three independent experiments. *, P < 0.05, for significant differences in levels of AI-2 production.