Effects of the luxS gene deletion on the biofilm formation of E. piscicida EIB202. Sterile supernatant at 10% was added to overnight cultures of the wild-type strain EIB202 at the beginning of culture. a, EIB202 at 6 h; b, ΔluxS strain at 6 h; c, ΔluxS strain with supernatant at 6 h; d, EIB202 at 12 h; e, ΔluxS strain at 12 h; f, ΔluxS strain with supernatant at 12 h; g, EIB202 at 24 h; h, ΔluxS strain at 24 h; i, ΔluxS strain with supernatant at 24 h.