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. 2020 Apr 20;88(5):e00002-20. doi: 10.1128/IAI.00002-20

FIG 3.

FIG 3

Determination of the capacity of the DU5883 strain complemented with pFnBR0, pFnBR1-11, or pFnBR0fnbA::sdsY (Hyb) to adhere to human fibronectin in vitro and to be internalized in MG63 cells. (A) Quantification of the fibronectin adhesion capacity of S. aureus DU5883 expressing pFnBR1-11 or pFnBR0fnbA::sdsY (Hyb). All results are expressed as the proportion of the values obtained for the S. aureus 8325-4 strain. The horizontal bars indicate the mean derived from three independent experiments performed in quadruplicate. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparison test using DU5883 pFnBPR0 as a control with an α risk of 0.05 (***, P ≤ 0.0001). (B) MG63 invasion by S. aureus DU5883 expressing pFnBR1-11 or pFnBR0fnbA::sdsY (Hyb). Internalized bacteria were measured as indicated above. Bars represent the mean ± standard deviation from three independent experiments performed in triplicate. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparison test using DU5883 pFnBPR0 as a control with an α risk of 0.05 (***, P ≤ 0.0001).