FIG 6.

RIP2-dependent PD-L1 induction by P. gingivalis. (A) SCC-25 cells were transfected with a plasmid encoding a RIP2-specific Cas9 enzyme cleaving in the promoter just upstream from the transcription start site and individual cell clones were tested for expression of RIP2 by Western blotting as shown. (B) Cells with wild-type and reduced levels of RIP2 were treated for 1 day with the indicated concentrations of P. gingivalis TM and analyzed by Western blotting for PD-L1 expression as shown. (C) The experiment was done as described for panel B except that C12-iE-DAP was used as a stimulating agent.