FIG 2.

Infection of CD18-deficient cells with bacteria elicits comparable bacterial association and cytotoxicity to WT cells. Flow cytometry to assay relative association of GFP-expressing PA14 WT or motABmotCD (A) or GFP-expressing PAO1 WT or fliC bacteria (B) with THP-1 WT or CD18 KO cells at 2 h postinfection (MOI of 25). The data are normalized to the mean fluorescence intensity (MFI) of uninfected THP-1 cells (set to 1,000). (C) Cytotoxicity of THP-1 WT or CD18 KO following infection with PA14 WT or motABmotCD (MOI of 25) was assayed using the LDH assay. Release of LDH into the culture supernatants was measured at 2 h postinfection. All data are analyzed using one-way ANOVA with Tukey’s post hoc analyses and are representative of at least two independent biological experiments (n ≥ 4). ***, P ≤ 0.0005; *, P ≤ 0.05; ns, not significant compared to WT cell type.