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. 2020 Apr 20;88(5):e00011-20. doi: 10.1128/IAI.00011-20

FIG 3.

FIG 3

N-glycans mediate P. aeruginosa association to THP-1 cells, and CR3 overexpression on CHO cells does not promote bacterial association. Relative association of GFP-expressing PAO1 WT (A) or PA14 WT (B) (MOI of 25 at 2 h postinfection) to THP-1 WT or CD18 KO cells without, or after, treatment of cells with 1 μg/ml tunicamycin. The data are normalized to the mean fluorescence intensity (MFI) of uninfected THP-1 cells analyzed by flow cytometry (set to 1,000). (C) Flow cytometry of Chinese hamster ovary (CHO) and CHO-CR3 (CD11b/CD18) cells stained with anti-human CD11b antibody. CHO is represented in blue and CHO-CR3 in magenta. (D) Relative association of GFP-expressing PAO1 WT or PA14 WT bacteria with CHO and CHO-CR3 cells, quantitatively measured by FACS analyses. The data are normalized to the mean fluorescence intensity (MFI) of uninfected CHO cells (set to 1,000). All data are analyzed using one-way ANOVA with Tukey’s post hoc analyses and are representative of at least two independent biological experiments (n ≥ 4). ***, P ≤ 0.0005; **, P ≤ 0.005; *, P ≤ 0.05; ns, not significant compared to WT cell type.