FIG 1.

Sae-mediated biofilm development in fetal bovine serum. GFP⁺ S. aureus strains LAC, UAMS-1, and Newman, including their respective isogenic mutants, were cultured statically in chambered cover glass at 37°C for 24 h in RPMI with/without 10% or 50% fetal bovine serum (FBS) or in 100% FBS. (A) Representative three-dimensional (3D) Z-stack confocal microscopy images of WT LAC biofilms developed in RPMI alone or with FBS as indicated. COMSTAT2 quantification of average biofilm thickness (in microns) and dimensionless roughness coefficients in LAC (B and C), UAMS-1 (D and E), and Newman (F and G) strains. Data are from three independent experiments and presented as means ± standard deviations (SDs). Statistical analyses were performed using a two-way analysis of variance (ANOVA) with Tukey’s post hoc multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, no statistically significant differences across the groups.