FIG 2.

FnBPA-dependent biofilm development in FBS and human serum (HS). GFP⁺ S. aureus strain LAC and its isogenic ΔfnbA and ΔfnbB mutants were cultured statically in chambered cover glass at 37°C for 24 h in RPMI with/without 10% or 50% FBS or in 100% FBS (A and B) or HS (C and D) followed by COMSTAT2 quantification of average biofilm thickness (in microns) and dimensionless roughness coefficients. Data are from three independent experiments and presented as means ±SDs. Statistical analyses were performed using a two-way ANOVA with Tukey’s post hoc multiple-comparison test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.