FIG 5.

FnBPA-mediated inhibition of macrophage biofilm invasion. CellTracker violet-labeled bone marrow-derived macrophage (MΦ) were added to WT LAC and ΔfnbA biofilms cultured in RPMI plus 50% FBS and assessed for MΦ invasion after 4 h. (A) Representative 3D Z-stack confocal images of BMDM (blue) invasion into LAC (top) and ΔfnbA (bottom) biofilms developed in RPMI plus 50% FBS. White arrows indicate BMDMs not clearly visible. (B) Quantified BMDM invasion as percentage of visible BMDMs in a sterile sample within the same x/y/z dimensions. Data are from three independent experiments and presented as means ± SDs. Statistical analyses were performed using a two-way ANOVA with Tukey’s post hoc multiple-comparison test. ****, P < 0.0001.