FIG 4.

The C. trachomatis tarP mutant is deficient in chlamydial entry (and the C-terminal F-actin-binding sites play a key role in invasion). Wild-type Chlamydia trachomatis (WT L2, black circles), C. trachomatis tarP mutant (Δtarp, red squares), and C. trachomatis tarP mutant complemented with pTarp (Δtarp + pTarp, green triangles), pTarpΔphos (Δtarp +pTarpΔphos, blue circles), pTarpΔPRD (Δtarp + pTarpΔPRD, yellow diamonds), pTarpΔABD (Δtarp + pTarpΔABD, purple circles), or pTarpΔFAB (Δtarp + pTarpΔFAB, black squares) were examined for chlamydial invasion of HeLa 229 cells after 1 h. The graph presented is from one representative experiment of three. Data sets were compared with one-way ANOVA and Tukey’s multiple comparison test of the mean. *, P < 0.05; ****, P < 0.0001.