FIG 6.

Replication of T. gondii is restricted by autophagy adaptors. (A) Wild-type HeLa cells and the indicated knockout cells were left unactivated or activated with IFN-γ for 24 h, infected with the parasite (MOI = 10), and harvested at 30 h postinfection. Infected cells were harvested and stained with anti-GRA7 and anti-SAG2 antibodies. T. gondii replication in vacuoles was counted by fluorescence microscopy. The average number of parasites per vacuole was determined from at least 100 vacuoles on three individual coverslips. Values are the mean ± SD of three independent experiments, and statistical analysis was conducted by two-way ANOVA; **** P < 0.0001. (B to E) HeLa and TKO cells were transfected with either empty pCAGGS vectors or plasmids expressing autophagy adaptors, left unactivated or activated with IFN-γ for 24 h, infected with the parasite (MOI = 10), and harvested at 30 h postinfection. Cells were then treated as above. The average number of parasites per vacuole was determined from at least 100 vacuoles on three individual coverslips. Values are the mean ± SD of three independent experiments, and statistical analysis was done by two-way ANOVA; *** P < 0.001, **** P < 0.0001.