FIG 4.
In vivo functionality of the OppABCDFCt transporter in C. trachomatis, as measured by growth arrest in the presence of TO. (A and B) Titer of C. trachomatis (IFU, inclusion-forming units) (A) and stained inclusions (B) after treatment with TO. HeLa cells were infected with C. trachomatis, and an anti-Chlamydia LPS antibody was used to visualize chlamydial inclusions. DAPI was used as a counterstain to visualize host cell nuclei. A reduction in titer represents the growth reduction of C. trachomatis. (C) Cytotoxicity assessment of HeLa cells for TO, as measured by the MTT assay. Data were evaluated by Student's t test (P < 0.05).