TABLE 5.
Primers used in strain construction
| Primer | Sequence (5′ to 3′)a | Restriction site |
|---|---|---|
| P1 | TCAATGCTTCACAATATACATAGTCCGACTG | SphI |
| P2 | TGATTAACTTTATAAGGAGGAAAAACATATGTCGGTTAGAGGGAAACTTATGAAG | SalI |
| P3 | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATTATGTTCGCTCTATCTTTT | KpnI |
| P4 | CTAGTGAAAGTGTGCATAGGTCAAATCAAC | PstI |
| P5 | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATCATCACAGGAAGTTTTTA | KpnI |
| P6 | CTATCCTTCAGCTAATGAAACTCTTTTTAAA | PstI |
| P7 | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATCGCAAGATATCAGTGGGA | KpnI |
| P8 | TTAAGAGTTTTTAGCATACCGCATATCA | SphI |
| P9 | TGATTAACTTTATAAGGAGGAAAAACATATGCTCAGCTACATAAAGAGGCGGCTGTT | KpnI |
| P10 | TCAAAAAGAGAAAGTAAAAGCTGGAATA | PstI |
| P11 | TGATTAACTTTATAAGGAGGAAAAACATATGACCAACATCACCAAGAGAAGTT | SalI |
| P12 | CTGCAGATAGTAATAAGAATAAATAGCGTCGGAATCG | PstR |
| P13 | ATTGTTAATAAAGTGAAAGCGC | EcoRV |
| P14 | TTAAAGCGGATCGACTTTCAGG | PstI |
a
Underlining indicates Shine-Dalgarno (S/D) sequences.