Figure 2.

iNOS‐inhibitor mediated effect on hypoxia‐induced HIF‐1α expression. A, Representative pictures of HIF‐1α staining in the ganglion cell layer. HIF‐1α⁺ cells are marked in green and cell nuclei in blue. The addition of CoCl₂ induced an increase or stabilization of the alpha subunit of the transcription factor. Under treatment with the iNOS inhibitor, the HIF‐1a levels were not lower compared with the CoCl₂ group after 4 d. In addition, at 8 d of cultivation, comparable amounts of HIF‐1α were detected in CoCl₂ and in the 1400W‐treated group. B, After 4 d, no significant differences in HIF‐1α mRNA expression were observed. At 8 d, the relative mRNA expression by CoCl₂ was twofold increased and could be lowered to the initial level by treatment with 1400W. Abbreviations: GCL, ganglion cell layer; IPL, inner plexiform layer. Scale bar = 20 µm. All data are shown as mean ± SEM; *P < .05