Figure 3.

mRNA expression of the HIF‐1α target genes. A, CoCl₂ had a significant effect on iNOS mRNA expression after 4 d and the additional treatment with 1400W only lead to a small decrease in mRNA. After 8 d, CoCl₂ induced a fourfold increase in iNOS mRNA expression, this could be prevented by the 1400W treatment. B, After 4 d, a massive increase in mRNA expression of HSP70 was observed in the CoCl₂ retinas, which was significantly reduced by treatment with 1400W. After 8 d, a significantly increased expression was still observed, but not as pronounced as at the previous time. However, the protective effect of the inhibitor was still detectable after 8 d. All data are shown as mean ± SEM; **P < .01