Figure 4.

Rescue of retinal ganglion cells after CoCl₂‐induced degeneration. A, Representative images of the immunohistological staining. RGCs were stained with an antibody against RBPMS (red) and cell nuclei with DAPI (blue). A significant loss of RGCs in the untreated degeneration groups (CoCl₂) was observed over the cultivation period of 4 and 8 d. B, After 4 d, neuroprotection of the RGCs was observed by treatment with the iNOS‐inhibitor compared with the CoCl₂ group. Even after 8 d of cultivation, a protection of the RGCs by 1400W could be noticed. The retinae of the treatment groups contained significantly more RGCs than the untreated retinae. GCL, ganglion cell layer; IPL, inner plexiform layer. Scale bar = 20 µm. All data are shown as mean ± SEM; *P < .05; **P < .01; ***P < .001